Protein Antibiotics: Ectolysins

What are Ectolysins?

Ectolysins are phage structural proteins, which enable phages to penetrate the bacterial cell. They cleave specific chemical bonds on the bacterial cell wall, leading to bacterial lysis and death.

Advantages of Ectolysins over Antibiotics

  • Rapid Action

    Ectolysins are rapid acting and cleave the cell wall within a few minutes of contact against antibiotics that only need a few hours to kill the cells.

  • Specific

    Ectolysins are very specific to the target bacterial species and hence kill only the target pathogen without affecting the microbiome or the commensal bacterial flora as compared to antibiotics which kill both the pathogens as well as the beneficial bacteria.

  • Mechanism of Action

    Ectolysins have novel mode of action- they include enzymatic mechanisms like Muramidase, Glucosaminidase, Amidase or endopeptidase depending upon the cell wall bond targeted by the individual ectolysin. The availability of a library of enzymatic domains offers the opportunity of protein engineering.

  • Bactericidal Activity Independence

    Bactericidal activity is not dependent on the metabolic stage of the cell – Ectolysins kill both actively growing as well as stationary phase cells irrespective of the metabolic state. But antibiotics act only on metabolically active cells.

  • Active on Biofilms

    Ectolysins are active both on planktonic bacteria & bacterial biofilms as against antibiotics, which act poorly on bacterial biofilms.

  • Low Propensity for Resistance

    Ectolysins cleave essential cell wall chemical bonds, which are highly conserved and omnipresent on the bacterial cell surface; hence the propensity for the development of bacterial resistance is low.

Ectolysins Against Gram Positive and Gram Negative Bacteria

gram-positive

Gram Positive

gram-negative

Gram Negative

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